Enhancement of MHC class I-restricted peptide-specific T cell induction by a DNA prime/MVA boost vaccination regime

Vaccine. 1998 Mar;16(5):439-45. doi: 10.1016/s0264-410x(97)00226-0.


Human immunodeficiency virus (HIV) vaccine candidates were previously constructed as a string of cytotoxic T lymphocyte (CTL) epitopes delivered and expressed using DNA and modified virus Ankara (MVA; an attenuated vaccinia virus) vectors. These vaccines were shown to induce interferon (IFN)-gamma-producing and cytolytic CD8+ T cells after a single vaccine administration. In the course of this work, immunization protocols were sought which would improve the levels of induced HIV-specific T cells. It was found that previous immunological exposure to MVA reduced the efficiency of subsequent priming and boosting using the same vaccine vehicle. However, a combined regime whereby the animals were first primed with the DNA vaccine and then boosted with MVA was the most potent protocol for the induction of both interferon-gamma-producing and cytolytic T cells against two CTL epitopes simultaneously. The general applicability of this novel vaccination method for induction of major histocompatibility complex class I-restricted T cells is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD8-Positive T-Lymphocytes / immunology
  • Cells, Cultured
  • DNA, Viral*
  • Epitopes
  • Female
  • Histocompatibility Antigens Class I / immunology*
  • Immunization, Secondary
  • Interferon Inducers
  • Interferon-gamma / biosynthesis
  • Mice
  • Mice, Inbred BALB C
  • Peptides / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*
  • Vaccines, Attenuated
  • Vaccinia virus / immunology*
  • Viral Vaccines*


  • DNA, Viral
  • Epitopes
  • Histocompatibility Antigens Class I
  • Interferon Inducers
  • Peptides
  • Vaccines, Attenuated
  • Viral Vaccines
  • Interferon-gamma