Replication profile of Saccharomyces cerevisiae chromosome VI

Genes Cells. 1997 Nov;2(11):667-78. doi: 10.1046/j.1365-2443.1997.1520350.x.


Background: An understanding of the replication programme at the genome level will require the identification and characterization of origins of replication through large, contiguous regions of DNA. As a step toward this goal, origin efficiencies and replication times were determined for 10 ARSs spanning most of the 270 kilobase (kb) chromosome VI of Saccharomyces cerevisiae.

Results: Chromosome VI shows a wide variation in the percentage of cell cycles in which different replication origins are utilized. Most of the origins are activated in only a fraction of cells, suggesting that the pattern of origin usage on chromosome VI varies greatly within the cell population. The replication times of fragments containing chromosome VI origins show a temporal pattern that has been recognized on other chromosomes--the telomeres replicate late in S phase, while the central region of the chromosome replicates early.

Conclusions: As demonstrated here for chromosome VI, analysis of the direction of replication fork movement along a chromosome and determination of replication time by measuring a period of hemimethylation may provide an efficient means of surveying origin activity over large regions of the genome.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Cycle / genetics*
  • Chromosome Mapping
  • Chromosomes, Fungal*
  • DNA Replication*
  • DNA, Fungal / analysis
  • DNA, Fungal / genetics
  • DNA, Fungal / metabolism
  • Genome, Fungal
  • Methylation
  • Polymerase Chain Reaction
  • Replication Origin*
  • S Phase / genetics
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Telomere / genetics
  • Time Factors


  • DNA, Fungal