Rb Interacts With Histone Deacetylase to Repress Transcription

Cell. 1998 Feb 20;92(4):463-73. doi: 10.1016/s0092-8674(00)80940-x.

Abstract

Previously, we found that Rb can actively repress transcription of cell cycle genes by binding and inactivating transcription factors at the promoter. Here, we demonstrate that Rb can also repress transcription of endogenous cell cycle genes containing E2F sites through recruitment of histone deacetylase, which deacetylates histones on the promoter, thereby promoting formation of nucleosomes that inhibit transcription. These two mechanisms of repression by Rb are selective-some promoters and transcription factors are blocked by this recruitment of histone deacetylase, whereas others are resistant to histone deacetylase activity and are repressed directly by inhibition of transcription factors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae / genetics
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Cell Cycle / genetics
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation, Enzymologic*
  • Gene Expression Regulation, Viral
  • Histone Deacetylases / chemistry
  • Histone Deacetylases / metabolism*
  • Humans
  • NF-kappa B / genetics
  • Osteoblasts / cytology
  • Osteoblasts / enzymology
  • Promoter Regions, Genetic / physiology
  • Protein Structure, Tertiary
  • Proto-Oncogene Proteins / genetics
  • Repressor Proteins*
  • Retinoblastoma Protein / chemistry
  • Retinoblastoma Protein / metabolism*
  • Simian virus 40 / genetics
  • Simplexvirus / genetics
  • Thymidine Kinase / genetics
  • Trans-Activators / genetics
  • Transcription Factor RelA
  • Transcription, Genetic / physiology*
  • Tumor Cells, Cultured / enzymology

Substances

  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • DNA-Binding Proteins
  • MXD1 protein, human
  • NF-kappa B
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • Retinoblastoma Protein
  • Trans-Activators
  • Transcription Factor RelA
  • proto-oncogene protein Spi-1
  • Thymidine Kinase
  • Histone Deacetylases