Human transferrin receptor is active and plasma membrane-targeted in yeast

FEMS Microbiol Lett. 1998 Mar 1;160(1):61-7. doi: 10.1111/j.1574-6968.1998.tb12891.x.

Abstract

The human transferrin receptor, a type II plasma membrane protein which mediates iron transport in human cells, was expressed in the yeast Saccharomyces cerevisiae. The transferrin receptor synthesized by yeast cells was posttranslationally modified comparable to the native receptor with respect to glycosylation and dimer formation. The location of the expressed receptor in the yeast plasma membrane indicates that the targeting of this type II membrane protein shares similar mechanisms in yeast and mammalian cells. The yeast-expressed transferrin receptor showed binding activity towards its natural ligand, transferrin in an ELISA binding assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Membrane / chemistry
  • Cell Membrane / metabolism*
  • Cloning, Molecular
  • DNA, Complementary
  • Gene Expression Regulation, Fungal
  • Humans
  • Microscopy, Immunoelectron
  • Protein Binding
  • Receptors, Transferrin / analysis
  • Receptors, Transferrin / genetics*
  • Receptors, Transferrin / metabolism*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae / ultrastructure
  • Subcellular Fractions / chemistry

Substances

  • DNA, Complementary
  • Receptors, Transferrin