Molecular characterization of a neuronal low-voltage-activated T-type calcium channel

Nature. 1998 Feb 26;391(6670):896-900. doi: 10.1038/36110.


The molecular diversity of voltage-activated calcium channels was established by studies showing that channels could be distinguished by their voltage-dependence, deactivation and single-channel conductance. Low-voltage-activated channels are called 'T' type because their currents are both transient (owing to fast inactivation) and tiny (owing to small conductance). T-type channels are thought to be involved in pacemaker activity, low-threshold calcium spikes, neuronal oscillations and resonance, and rebound burst firing. Here we report the identification of a neuronal T-type channel. Our cloning strategy began with an analysis of Genbank sequences defined as sharing homology with calcium channels. We sequenced an expressed sequence tag (EST), then used it to clone a full-length complementary DNA from rat brain. Northern blot analysis indicated that this gene is expressed predominantly in brain, in particular the amygdala, cerebellum and thalamus. We mapped the human gene to chromosome 17q22, and the mouse gene to chromosome 11. Functional expression of the channel was measured in Xenopus oocytes. Based on the channel's distinctive voltage dependence, slow deactivation kinetics, and 7.5-pS single-channel conductance, we conclude that this channel is a low-voltage-activated T-type calcium channel.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Calcium Channels / genetics*
  • Calcium Channels / metabolism
  • Calcium Channels, L-Type
  • Cells, Cultured
  • Chromosome Mapping
  • Chromosomes, Human, Pair 17
  • Cloning, Molecular
  • DNA, Complementary
  • Databases, Factual
  • Electrophysiology
  • Humans
  • Ion Channel Gating*
  • Molecular Sequence Data
  • Muscle, Skeletal / metabolism
  • Mutation
  • Rats
  • Sequence Alignment
  • Xenopus


  • Calcium Channels
  • Calcium Channels, L-Type
  • DNA, Complementary

Associated data

  • GENBANK/AF027984
  • GENBANK/AF029228
  • GENBANK/AF029229