Cloning and characterization of transcription of the xylAB operon in Thermoanaerobacter ethanolicus

J Bacteriol. 1998 Mar;180(5):1103-9. doi: 10.1128/JB.180.5.1103-1109.1998.

Abstract

The genes encoding xylose isomerase (xylA) and xylulose kinase (xylB) from the thermophilic anaerobe Thermoanaerobacter ethanolicus were found to constitute an operon with the transcription initiation site 169 nucleotides upstream from the previously assigned (K. Dekker, H. Yamagata, K. Sakaguchi, and S. Udaka, Agric. Biol. Chem. 55:221-227, 1991) promoter region. The bicistronic xylAB mRNA was processed by cleavage within the 5'-terminal portion of the XylB-coding sequence. Transcription of xylAB was induced in the presence of xylose, and, unlike in all other xylose-utilizing bacteria studied, was not repressed by glucose. The existence of putative xyl operator sequences suggested that xylose utilization is controlled by a repressor-operator mechanism. The T. ethanolicus xylB gene coded for a 500-amino-acid-residue protein with a deduced amino acid sequence highly homologous to those of other XylBs. This is the first report of an xylB nucleotide sequence and an xyLAB operon from a thermophilic anaerobic bacterium.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aldose-Ketose Isomerases / genetics*
  • Aldose-Ketose Isomerases / metabolism
  • Amino Acid Sequence
  • Bacteria, Anaerobic / enzymology
  • Bacteria, Anaerobic / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Gene Expression Regulation, Bacterial
  • Gram-Positive Asporogenous Rods, Irregular / enzymology
  • Gram-Positive Asporogenous Rods, Irregular / genetics*
  • Molecular Sequence Data
  • Operator Regions, Genetic
  • Operon*
  • Phosphotransferases (Alcohol Group Acceptor) / genetics*
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism
  • RNA, Bacterial / genetics
  • RNA, Bacterial / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Regulatory Sequences, Nucleic Acid
  • Sequence Alignment
  • Transcription, Genetic*
  • Xylose / metabolism
  • Xylulose / metabolism

Substances

  • RNA, Bacterial
  • RNA, Messenger
  • Xylulose
  • Xylose
  • Phosphotransferases (Alcohol Group Acceptor)
  • xylulokinase
  • Aldose-Ketose Isomerases
  • xylose isomerase

Associated data

  • GENBANK/AF001974