Porcine Reproductive and Respiratory Syndrome Virus Infection of Gnotobiotic Pigs: Sites of Virus Replication and Co-Localization With MAC-387 Staining at 21 Days Post-Infection

Virus Res. 1997 Oct;51(2):105-13. doi: 10.1016/s0168-1702(97)00086-5.


The organ distribution of PRRSV-infected cells in gnotobiotic piglets at 21 days after infection with PRRSV isolate VR-2332 was examined by in situ hybridization. Cells that expressed PRRSV RNA were identified in all tissues examined, including organs not usually characterized as sites of PRRSV infection. PRRSV-infected cells frequently appeared in clusters and were not always associated with microscopic lesions. The expression of PRRSV RNA co-localized with a macrophage monoclonal antibody, MAC-387, in lymph nodes. Some, but not all infected cells stained with MAC-387. The wide distribution of PRRSV-infected cells and co-localization with MAC-387 staining is consistent with the macrophage-tropism of PRRSV and is similar to observations made during persistent infection with other arteriviruses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Germ-Free Life*
  • In Situ Hybridization
  • Macrophages / immunology
  • Macrophages / virology*
  • Porcine Reproductive and Respiratory Syndrome / pathology
  • Porcine Reproductive and Respiratory Syndrome / virology*
  • RNA, Viral
  • Staining and Labeling
  • Swine
  • Virus Replication


  • Antibodies, Monoclonal
  • RNA, Viral