Fast screening method for the profile analysis of polycyclic aromatic hydrocarbon metabolites in urine using derivatisation--solid-phase microextraction

J Chromatogr B Biomed Sci Appl. 1998 Jan 23;705(1):132-8. doi: 10.1016/s0378-4347(97)00526-4.


A method for the qualitative analysis of various metabolites of naphthalene, phenanthrene and pyrene is presented. The method uses SPME with a 85-microm polyacrylate fibre for extraction, headspace silylation with BSTFA without any catalyst for on-fibre derivatisation and GC-MS in the SIR mode for separation and detection. The suitability of the method for profile analysis of PAH metabolites is shown by analysing a smokers urine after enzymatic cleavage (and additionally after spiking with the target analytes) and spiked water. The method exhibits satisfactory separation of all investigated metabolites and no interferences due to matrix peaks.

MeSH terms

  • Acrylic Resins
  • Carcinogens, Environmental / analysis*
  • Gas Chromatography-Mass Spectrometry / methods
  • Humans
  • Indicators and Reagents
  • Naphthalenes / urine
  • Phenanthrenes / urine
  • Polycyclic Aromatic Hydrocarbons / urine*
  • Pyrenes / analysis
  • Smoking / urine
  • Trimethylsilyl Compounds


  • Acrylic Resins
  • Carcinogens, Environmental
  • Indicators and Reagents
  • Naphthalenes
  • Phenanthrenes
  • Polycyclic Aromatic Hydrocarbons
  • Pyrenes
  • Trimethylsilyl Compounds
  • carbopol 940
  • N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide