TGF-beta1 produced by gastric cancer cells affects mesothelial cell morphology in peritoneal dissemination

Int J Oncol. 1998 Apr;12(4):847-51. doi: 10.3892/ijo.12.4.847.

Abstract

In vitro morphologic change of mesothelial cells was observed following the addition of serum-free conditioned medium (SF-CM) from peritoneal dissemination cell line OCUM-2MD3. The same morphologic change of mesothelial cells was observed following the addition of 10 ng/ml TGF-beta1, but not following the addition of b-FGF, IGF-I, VEGF or PDGF-AA. In the in vivo study, mesothelial cells of mice treated with SF-CM from OCUM-2MD3 and TGF- beta1 were separated from one another, resulting in exposure of the submesothelial connective tissue. The molecular size of the mesothelial morphology changing activity was estimated by running the SF-CM from OCUM-2MD3 through a gel filtration column TSK-gel G2000SW. The mesothelial morphology changing activity was recognized at positions equivalent of Mr 6, 500-30,000. 25 kDa TGF-beta1 was detected in the active fraction from the TSK-gel G2000SW column and the SF-CM of OCUM-2MD3 by Western blotting using a monoclonal antibody against TGF-beta1. These findings suggest that TGF-beta1 produced by gastric cancer cells changes the morphology of mesothelial cells and may thus be closely associated with peritoneal dissemination.

MeSH terms

  • Animals
  • Epithelium / pathology
  • Female
  • Humans
  • Mice
  • Mice, Nude
  • Molecular Weight
  • Peritoneal Neoplasms / secondary*
  • Stomach Neoplasms / pathology*
  • Transforming Growth Factor beta / physiology*
  • Tumor Cells, Cultured

Substances

  • Transforming Growth Factor beta