Comparative analysis of expression of hepatocyte growth factor and its receptor, c-met, in gliomas, meningiomas and schwannomas in humans

Cancer Lett. 1998 Feb 27;124(2):149-55. doi: 10.1016/s0304-3835(97)00469-2.

Abstract

Expression of hepatocyte growth factor (HGF) and c-met, a proto-oncogene that encodes a receptor for HGF, was examined in 45 cases of human primary intracranial tumors by means of RT-PCR. In gliomas, HGF and c-met mRNAs were preferentially expressed in high-grade tumors. Co-expression of both genes was observed in glioblastomas (6/15) and in one anaplastic astrocytoma (1/5) but not in low-grade astrocytomas (0/3). By contrast, the c-met gene was consistently expressed in meningiomas (12/14) and schwannomas (8/8). The presence of c-Met protein was confirmed in the tumor cells of glioblastoma, meningioma and schwannoma by immunohistochemical staining. Moreover, all of the schwannoma cases co-expressed the HGF gene. These observations suggest that HGF/c-met expression is somehow related to the disease progression in gliomas, whereas c-Met protein might have an important fundamental biological role in meningioma and schwannoma. Moreover, since all of the schwannoma cases concomitantly expressed the ligand (HGF) and the receptor (c-met) genes, HGF may act in an autocrine fashion in schwannoma.

Publication types

  • Comparative Study

MeSH terms

  • Astrocytoma / metabolism*
  • Astrocytoma / pathology
  • Disease Progression
  • Gene Expression
  • Glioblastoma / metabolism*
  • Glioblastoma / pathology
  • Hepatocyte Growth Factor / biosynthesis*
  • Humans
  • Immunohistochemistry
  • Meningioma / metabolism*
  • Neurilemmoma / metabolism*
  • Neurilemmoma / pathology
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins c-met / biosynthesis*
  • Transcription, Genetic

Substances

  • Hepatocyte Growth Factor
  • Proto-Oncogene Proteins c-met