Single-strand conformation polymorphism (SSCP) analysis of Listeria monocytogenes iap gene as tool to detect different serogroups

Mol Cell Probes. 1997 Dec;11(6):459-62. doi: 10.1006/mcpr.1997.0137.


PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a convenient technique for the detection of mutations. As the mobility of single-stranded DNA is sequence-dependent it could therefore be used to determine serotype-related sequence variations in Listeria monocytogenes. Sero-specific patterns were observed in different L. monocytogenes serogroups.

MeSH terms

  • Bacterial Proteins / genetics*
  • DNA Primers
  • Genetic Variation
  • Humans
  • Listeria monocytogenes / classification*
  • Listeria monocytogenes / genetics*
  • Polymerase Chain Reaction / methods
  • Polymorphism, Single-Stranded Conformational*
  • Reproducibility of Results
  • Serotyping / methods


  • 60 kDa protein, Listeria monocytogenes
  • Bacterial Proteins
  • DNA Primers