Cytokine induced expression of porcine inhibitor of apoptosis protein (iap) family member is regulated by NF-kappa B

Biochem Biophys Res Commun. 1998 Feb 24;243(3):827-32. doi: 10.1006/bbrc.1998.8185.


The inhibitor of apoptosis (iap) proteins belong to a gene family that protect certain cell to undergo programmed cell death in response to a variety of stimuli. By differential screening we have identified a cDNA clone, designated piap, in porcine aortic endothelial cells (PAEC) that turned out by sequence comparison to be a porcine member of the iap family. The expression of piap is strongly up-regulated upon treatment of endothelial cells (EC) with inflammatory cytokines TNF-alpha, IL-1 beta, and LPS. In EC these stimuli lead to the activation of nuclear transcription factor kappa B (NF-kappa B) that plays a role in countering TNF-alpha induced apoptosis. We demonstrate that adenovirus mediated overexpression of I kappa B alpha, an inhibitor of NF-kappa B suppresses the expression of piap in response to TNF-alpha suggesting that piap is one of the NF-kappa B regulated genes that operates to prevent programmed cell death of EC in inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aorta / chemistry
  • Apoptosis / genetics*
  • Apoptosis Regulatory Proteins
  • Base Sequence
  • Cells, Cultured
  • Consensus Sequence
  • Cytokines / pharmacology*
  • DNA-Binding Proteins / pharmacology
  • Endothelium, Vascular / chemistry
  • Gene Expression*
  • I-kappa B Proteins*
  • Interleukin-1 / pharmacology
  • Lipopolysaccharides / pharmacology
  • Molecular Sequence Data
  • NF-KappaB Inhibitor alpha
  • NF-kappa B / antagonists & inhibitors
  • NF-kappa B / pharmacology*
  • Proteins / genetics*
  • Swine
  • Tumor Necrosis Factor-alpha / pharmacology


  • Apoptosis Regulatory Proteins
  • Cytokines
  • DNA-Binding Proteins
  • I-kappa B Proteins
  • Interleukin-1
  • Lipopolysaccharides
  • NF-kappa B
  • Proteins
  • Tumor Necrosis Factor-alpha
  • apoptosis protein inhibitor, porcine
  • NF-KappaB Inhibitor alpha

Associated data

  • GENBANK/U79142