Targeted disruption of the ubiquitous CNC-bZIP transcription factor, Nrf-1, results in anemia and embryonic lethality in mice

EMBO J. 1998 Mar 16;17(6):1779-87. doi: 10.1093/emboj/17.6.1779.

Abstract

The CNC-basic leucine zipper (CNC-bZIP) family is a subfamily of bZIP proteins identified from independent searches for factors that bind the AP-1-like cis-elements in the beta-globin locus control region. Three members, p45-Nf-e2, Nrf-1 and Nrf-2 have been identified in mammals. Expression of p45-Nf-e2 is largely restricted to hematopoietic cells while Nrf-1 and Nrf-2 are expressed in a wide range of tissues. To determine the function of Nrf-1, targeted disruption of the Nrf-1 gene was carried out. Homozygous Nrf-1 mutant mice are anemic due to a non-cell autonomous defect in definitive erythropoiesis and die in utero.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Anemia / genetics*
  • Animals
  • DNA-Binding Proteins / analysis
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Embryonic and Fetal Development
  • Erythropoiesis / genetics*
  • Fetus
  • Genes, Lethal / physiology*
  • Homozygote
  • Leucine Zippers
  • Liver
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • NF-E2-Related Factor 1
  • Nuclear Respiratory Factor 1
  • Nuclear Respiratory Factors
  • RNA, Messenger / analysis
  • Trans-Activators / analysis
  • Trans-Activators / genetics
  • Trans-Activators / physiology*

Substances

  • DNA-Binding Proteins
  • NF-E2-Related Factor 1
  • Nrf1 protein, mouse
  • Nuclear Respiratory Factor 1
  • Nuclear Respiratory Factors
  • RNA, Messenger
  • Trans-Activators