Interferon-beta Is Required for Interferon-Alpha Production in Mouse Fibroblasts

Curr Biol. 1998 Feb 12;8(4):223-6. doi: 10.1016/s0960-9822(98)70086-7.

Abstract

The type I interferons--interferon-alpha (IFN-alpha) and interferon-beta (IFN-beta)--are critical for protection against viruses during the acute stage of viral infection [1,2]. Furthermore, type I interferons have been implicated as important mediators in the regulation of lymphocyte development [3], immune responses [4,5] and the maintenance of immunological memory of cytotoxic T cells [6,7]. The different IFN-alpha subtypes are encoded by 12 genes in the mouse [8] whereas IFN-beta is encoded by only one gene [9]. IFN-alpha and IFN-beta have a high degree of sequence homology and are thought to interact with the same surface receptor on target cells [10,11]. As an approach to analysing the different biological functions of IFN-alpha and IFN-beta, we have generated a mouse strain with an inactivated IFN-beta gene. We report here that embryonic fibroblasts from such mice produce neither IFN-beta nor IFN-alpha upon Sendal virus infection, whereas the production of IFN-alpha by leukocytes from the same strain of mice is intact. IFN-alpha production in embryonic fibroblasts from IFN-beta-/- mice could be rescued by 'priming' the cells using exogenous IFN-beta. These results imply a unique role for IFN-beta in the induction of type I interferons in peripheral tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Embryo, Mammalian / cytology
  • Embryo, Mammalian / metabolism*
  • Embryo, Mammalian / virology
  • Fibroblasts / metabolism
  • Fibroblasts / virology
  • Interferon-alpha / biosynthesis*
  • Interferon-beta / genetics
  • Interferon-beta / physiology*
  • Mice
  • Mutagenesis
  • Respirovirus / physiology

Substances

  • Interferon-alpha
  • Interferon-beta