Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
, 110 Suppl 1, 59P-62P

[Production of Recombinant eCG With Potent FSH-like Activity by Site-Directed Mutagenesis]

[Article in Japanese]
Affiliations
Review

[Production of Recombinant eCG With Potent FSH-like Activity by Site-Directed Mutagenesis]

[Article in Japanese]
K Shiota et al. Nihon Yakurigaku Zasshi.

Abstract

Equine chorionic gonadotropin (eCG) and luteinizing hormone (eLH) are encoded by a single gene and have identical peptide portions. They, however, differ in the structures of their attached oligosaccharides, which depend on the sites of their production. Recombinant eCG/LH (rec-eCG/LH) possesses dual LH and FSH activities. Mutant eCG/LH in which Asn 56 of the alpha-subunit was changed to Gln to remove the N-linked oligosaccharide showed complete loss of LH activity, whereas in contrast its FSH activity was more potent than that of the wild-type. Another mutant, which lacked the carboxyterminal portion of the beta-subunit to which O-linked oligosaccharides are attached, showed LH activity similar to that of the wild-type, whereas it had the most potent FSH activity. Thus, the oligosaccharides attached to eCG/LH play differential roles in the expression of biological activity.

Similar articles

See all similar articles

LinkOut - more resources

Feedback