siaD PCR ELISA for confirmation and identification of serogroup Y and W135 meningococcal infections

FEMS Microbiol Lett. 1998 Feb 15;159(2):209-14. doi: 10.1111/j.1574-6968.1998.tb12862.x.


Non-culture diagnosis and serogroup determination of meningococcal infection is important in contact management where vaccination may be possible. A serogroup B and C PCR ELISA assay for the non-culture diagnosis and serogroup determination has proved invaluable for enhanced epidemiological surveillance and contact management. A polymerase chain reaction assay, based on a restriction fragment length polymorphism in the meningococcal serogroup Y and W135 sialyltransferase (siaD) gene, was developed to enhance the range of non-culture diagnosis of meningococcal infection from clinical samples. The PCR assay was adapted to an ELISA format incorporating hybridisation with serogroup-specific Y and W135 oligonucleotide probes. The serogroup-specific W135 and Y PCR ELISA is a useful addition to currently available serogroup B and C assay for non-culture diagnosis of meningococcal infection and outbreak investigation.

MeSH terms

  • Enzyme-Linked Immunosorbent Assay
  • Genes, Bacterial*
  • Meningococcal Infections / diagnosis*
  • Neisseria meningitidis / classification*
  • Neisseria meningitidis / genetics
  • Polymerase Chain Reaction*
  • Polymorphism, Restriction Fragment Length
  • Serotyping
  • Sialyltransferases / genetics*


  • Sialyltransferases