High pressure induces G2 arrest in murine erythroleukemia cells

J Biochem. 1998 Jan;123(1):87-93. doi: 10.1093/oxfordjournals.jbchem.a021920.

Abstract

The effect of high pressure on proliferation and cell cycle progression was examined using murine erythroleukemia (MEL) cells. The MEL cells were exposed to high pressures (0.1-130 MPa) and then cultured for 5 days at atmospheric pressure. The proliferation of MEL cells was unaffected up to 60 MPa, but was suppressed at 80-110 MPa. Above 120 MPa, the cells were fragmented. The cell cycle analysis of 80 MPa-treated MEL cells showed that the cells in S phase are most sensitive to high pressure and they arrest in G2 phase. Interestingly, G2-arrested cells reinitiated DNA synthesis, resulting in giant cells with high DNA contents. Furthermore, when such G2-arrested cells were exposed to caffeine, premature mitosis, characterized by chromosome pulverization, was observed. These results suggest that the suppression of proliferation in high-pressure-treated MEL cells is associated with G2 arrest following S phase delay. Thus, it seems valuable to apply high pressure to the investigation of the cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine / analogs & derivatives
  • Adenine / pharmacology
  • Animals
  • Caffeine / pharmacology
  • Cell Cycle / drug effects
  • Cell Division*
  • G2 Phase*
  • Leukemia, Erythroblastic, Acute
  • Mice
  • Mitotic Index
  • Pressure*
  • Tumor Cells, Cultured

Substances

  • Caffeine
  • N(6),N(6)-dimethyladenine
  • Adenine