Organelle membrane fusion: a novel function for the syntaxin homolog Ufe1p in ER membrane fusion

Cell. 1998 Mar 6;92(5):611-20. doi: 10.1016/s0092-8674(00)81129-0.

Abstract

The fusion of endoplasmic reticulum (ER) membranes in yeast does not require Sec18p/NSF and Sec17p, two proteins needed for docking of vesicles with their target membrane. Instead, ER membranes require a NSF-related ATPase, Cdc48p. Since both vesicular and organelle fusion events use related ATPases, we investigated whether both fusion events are also SNARE mediated. We present evidence that the fusion of ER membranes requires Ufe1p, a t-SNARE that localizes to the ER, but no known v-SNAREs. We propose that the Ufe1 protein acts in the dual capacity of an organelle membrane fusion-associated SNARE by undergoing direct t-t-SNARE and Cdc48p interactions during organelle membrane fusion as well as a t-SNARE for vesicular traffic.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases
  • Adenosine Triphosphate / metabolism
  • Carrier Proteins*
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism
  • Cell Cycle Proteins / physiology
  • Endoplasmic Reticulum / metabolism*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Fungal Proteins / physiology*
  • Glycoproteins*
  • Intracellular Membranes
  • Membrane Fusion / physiology*
  • Membrane Glycoproteins / physiology
  • Membrane Proteins / analysis
  • Membrane Proteins / physiology
  • Microsomes / metabolism
  • Mutation
  • Peptide Fragments
  • Qa-SNARE Proteins
  • Qb-SNARE Proteins
  • Recombinant Fusion Proteins
  • SNARE Proteins
  • Saccharomyces cerevisiae Proteins*
  • Valosin Containing Protein
  • Vesicular Transport Proteins*
  • Yeasts / cytology

Substances

  • Carrier Proteins
  • Cell Cycle Proteins
  • Fungal Proteins
  • Glycoproteins
  • Membrane Glycoproteins
  • Membrane Proteins
  • Peptide Fragments
  • Qa-SNARE Proteins
  • Qb-SNARE Proteins
  • Recombinant Fusion Proteins
  • SEC20 protein, S cerevisiae
  • SNARE Proteins
  • Saccharomyces cerevisiae Proteins
  • TIP20 protein, S cerevisiae
  • UFE1 protein, S cerevisiae
  • Vesicular Transport Proteins
  • Adenosine Triphosphate
  • Adenosine Triphosphatases
  • CDC48 protein, S cerevisiae
  • Valosin Containing Protein