Practical approach for detection and identification of OXA-10-derived ceftazidime-hydrolyzing extended-spectrum beta-lactamases

J Clin Microbiol. 1998 Mar;36(3):827-9. doi: 10.1128/JCM.36.3.827-829.1998.

Abstract

A practical approach to detect and identify ceftazidime-hydrolyzing extended-spectrum mutants of OXA-10 beta-lactamase is presented. Large numbers of bacteria were screened by colony hybridization, a 720-bp part of blaOXA was amplified by PCR from the hybridization-positive isolates, and the products were digested by PvuII and HaeIII.

MeSH terms

  • Ceftazidime / metabolism*
  • Ceftazidime / pharmacology
  • Cephalosporin Resistance / genetics
  • Cephalosporins / metabolism*
  • Cephalosporins / pharmacology
  • Humans
  • Mutation
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction
  • Pseudomonas Infections / microbiology
  • Pseudomonas aeruginosa / drug effects
  • Pseudomonas aeruginosa / enzymology*
  • Pseudomonas aeruginosa / genetics
  • beta-Lactamases / analysis*
  • beta-Lactamases / genetics
  • beta-Lactamases / metabolism

Substances

  • Cephalosporins
  • Ceftazidime
  • beta-lactamase PSE-2
  • beta-Lactamases