Abstract
A practical approach to detect and identify ceftazidime-hydrolyzing extended-spectrum mutants of OXA-10 beta-lactamase is presented. Large numbers of bacteria were screened by colony hybridization, a 720-bp part of blaOXA was amplified by PCR from the hybridization-positive isolates, and the products were digested by PvuII and HaeIII.
MeSH terms
-
Ceftazidime / metabolism*
-
Ceftazidime / pharmacology
-
Cephalosporin Resistance / genetics
-
Cephalosporins / metabolism*
-
Cephalosporins / pharmacology
-
Humans
-
Mutation
-
Nucleic Acid Hybridization
-
Polymerase Chain Reaction
-
Pseudomonas Infections / microbiology
-
Pseudomonas aeruginosa / drug effects
-
Pseudomonas aeruginosa / enzymology*
-
Pseudomonas aeruginosa / genetics
-
beta-Lactamases / analysis*
-
beta-Lactamases / genetics
-
beta-Lactamases / metabolism
Substances
-
Cephalosporins
-
Ceftazidime
-
beta-lactamase PSE-2
-
beta-Lactamases