Pre-MBT patterning of early gene regulation in Xenopus: the role of the cortical rotation and mesoderm induction

Mech Dev. 1998 Jan;70(1-2):15-24. doi: 10.1016/s0925-4773(97)00163-9.

Abstract

Patterning events that occur before the mid-blastula transition (MBT) and that organize the spatial pattern of gene expression in the animal hemisphere have been analyzed in Xenopus embryos. We present evidence that genes that play a role in dorsoventral specification display different modes of activation. Using early blastomere explants (16-128-cell stage) cultured until gastrula stages, we demonstrate by RT-PCR analysis that the expression of goosecoid (gsc), wnt-8 and brachyury (bra) is dependent on mesoderm induction. In contrast, nodal-related 3 (nr3) and siamois (sia) are expressed in a manner that is independent of mesoderm induction, however their spatially correct activation does require cortical rotation. The pattern of sia and nr3 expression reveals that the animal half of the 16-cell embryo is already distinctly polarized along the dorsoventral axis as a result of rearrangement of the egg structure during cortical rotation. Similar to the antagonistic activity between the ventral and the dorsal mesoderm, the ventral animal blastomeres can attenuate the expression of nr3 and sia in dorsal animal blastomeres. Our data suggest that no Nieuwkoop center activity at the blastula stage is required for the activation of nr3 and sia in vivo.

MeSH terms

  • Animals
  • Base Sequence
  • Blastocyst / cytology
  • Blastocyst / metabolism
  • Blastomeres / cytology
  • Blastomeres / metabolism
  • Body Patterning
  • Cytoskeletal Proteins
  • DNA Primers / genetics
  • DNA-Binding Proteins / genetics
  • Fetal Proteins*
  • Gene Expression Regulation, Developmental*
  • Goosecoid Protein
  • Homeodomain Proteins / genetics
  • Mesoderm / cytology
  • Mesoderm / metabolism
  • Polymerase Chain Reaction
  • Proteins / genetics
  • Repressor Proteins*
  • T-Box Domain Proteins*
  • Transcription Factors / genetics
  • Wnt Proteins
  • Xenopus / embryology*
  • Xenopus / genetics*
  • Xenopus Proteins
  • Zebrafish Proteins

Substances

  • Cytoskeletal Proteins
  • DNA Primers
  • DNA-Binding Proteins
  • Fetal Proteins
  • Goosecoid Protein
  • Homeodomain Proteins
  • Proteins
  • Repressor Proteins
  • SIA1 protein, Xenopus
  • T-Box Domain Proteins
  • Transcription Factors
  • Wnt Proteins
  • Xenopus Proteins
  • Zebrafish Proteins
  • wnt8a protein, zebrafish
  • Brachyury protein