A mouse Sertoli cell line expressing anti-Müllerian hormone and its type II receptor

Mol Cell Endocrinol. 1997 Dec 31;136(1):57-65. doi: 10.1016/s0303-7207(97)00214-1.


Anti-Müllerian hormone (AMH) induces the regression of Müllerian ducts in the male foetus; it is secreted by prepubertal testicular Sertoli cells and repressed at puberty. Using an AMH promoter/Simian virus 40 (SV40) oncogene fusion gene, we generated transgenic mouse lines exhibiting heritable Sertoli cell tumorigenesis. One cell line, derived from an adult male, expressed mRNAs characteristic of mature Sertoli cells, but no AMH. Two other cell lines were obtained from pretumoral testes at 6.5 days. One was cloned to yield SMAT1, whose expression pattern was characteristic of prepubertal Sertoli cells, namely no transferrin and high SF-I and AMH expression. SMAT1 also secretes AMH protein into the culture medium and expresses the AMH receptor. To the best of our knowledge, this is the first Sertoli cell line stably expressing AMH and its receptor. Our results show that, in targeted oncogenesis, the timing of cell line derivation plays a critical role even when using a developmentally regulated promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Mullerian Hormone
  • Antigens, Polyomavirus Transforming / genetics
  • Cell Line
  • DNA, Recombinant / genetics
  • Female
  • Gene Expression
  • Glycoproteins*
  • Growth Inhibitors / genetics
  • Growth Inhibitors / metabolism*
  • Humans
  • Male
  • Mice
  • Mice, Transgenic
  • Oncogenes / genetics
  • Ovarian Neoplasms / genetics
  • Promoter Regions, Genetic / genetics
  • RNA, Messenger / analysis
  • RNA, Viral / analysis
  • Receptors, Peptide / metabolism*
  • Receptors, Transforming Growth Factor beta
  • Sertoli Cell Tumor / genetics
  • Sertoli Cells / metabolism*
  • Simian virus 40 / genetics
  • Simian virus 40 / immunology
  • Testicular Hormones / genetics
  • Testicular Hormones / metabolism*
  • Testicular Neoplasms / genetics
  • Testicular Neoplasms / pathology
  • Testis / chemistry
  • Tumor Cells, Cultured


  • Antigens, Polyomavirus Transforming
  • DNA, Recombinant
  • Glycoproteins
  • Growth Inhibitors
  • RNA, Messenger
  • RNA, Viral
  • Receptors, Peptide
  • Receptors, Transforming Growth Factor beta
  • Testicular Hormones
  • anti-Mullerian hormone receptor
  • Anti-Mullerian Hormone