Putative phospholipid hydroperoxide glutathione peroxidase gene from Arabidopsis thaliana induced by oxidative stress

Genes Genet Syst. 1997 Oct;72(5):311-6. doi: 10.1266/ggs.72.311.

Abstract

An Arabidopsis cDNA encoding putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was cloned and sequenced. The cDNA comprised 803 bp and included an open reading frame which encodes a polypeptide of 169 amino acid residues. The deduced amino acid sequence showed about 80 and 50% homology with plant putative PHGPXs and mammalian PHGPXs, respectively. Southern blot analysis suggested that putative PHGPX gene was a single-copy gene. The expression profile of the putative PHGPX in Arabidopsis under NaCl and Al/Fe treatments, which generate oxidative stress, was analyzed. Northern blot analysis revealed that the Arabidopsis putative PHGPX mRNA levels were increased about 3 and 4.5 times after exposure to NaCl and Al/Fe, respectively. These results suggest that the putative PHGPX gene is induced by oxidative stress in Arabidopsis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aluminum / pharmacology
  • Amino Acid Sequence
  • Arabidopsis / enzymology
  • Arabidopsis / genetics*
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Complementary
  • Gene Expression Regulation, Plant / drug effects
  • Glutathione Peroxidase / drug effects
  • Glutathione Peroxidase / genetics*
  • Glutathione Peroxidase / metabolism
  • Iron / pharmacology
  • Molecular Sequence Data
  • Oxidative Stress*
  • Phospholipid Hydroperoxide Glutathione Peroxidase
  • Sequence Homology, Amino Acid
  • Sodium Chloride / pharmacology

Substances

  • DNA, Complementary
  • Sodium Chloride
  • Aluminum
  • Iron
  • Phospholipid Hydroperoxide Glutathione Peroxidase
  • Glutathione Peroxidase

Associated data

  • GENBANK/AB001568