Abstract
We cloned a genomic fragment of the 5'-flanking region of the gene encoding bone morphogenetic protein-6 (BMP-6) and assessed its promoter activity. Primer extension revealed the presence of one major transcription start site 178 bp upstream of the translation start site. The promoter region lacked a canonical TATA box but did contain a GC-rich region. A putative tramtrack responsive element, a Drosophila transcriptional factor regulating the segment polarity, was found in the promoter region. Known steroid hormonal responsive elements, however, were not found. Although BMP-6 is classified as a member of the vgr-1 family, the structure of the promoter was similar to that of BMP-2 and 4.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Bone Morphogenetic Protein 6
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Bone Morphogenetic Proteins / biosynthesis*
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Bone Morphogenetic Proteins / genetics*
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Cloning, Molecular / methods
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Fibrosarcoma
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Genes, Reporter
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Humans
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Luciferases / biosynthesis
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Molecular Sequence Data
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Osteosarcoma
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Polymerase Chain Reaction
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Promoter Regions, Genetic*
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Protein Biosynthesis
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Recombinant Fusion Proteins / biosynthesis
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Regulatory Sequences, Nucleic Acid*
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Transcription, Genetic
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Transfection
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Transforming Growth Factor beta / biosynthesis
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Transforming Growth Factor beta / genetics
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Tumor Cells, Cultured
Substances
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BMP6 protein, human
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Bone Morphogenetic Protein 6
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Bone Morphogenetic Proteins
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Recombinant Fusion Proteins
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Transforming Growth Factor beta
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Luciferases