Purification of the alkaloid lycorine and simultaneous analysis of ascorbic acid and lycorine by micellar electrokinetic capillary chromatography

Anal Biochem. 1998 Mar 1;257(1):80-8. doi: 10.1006/abio.1997.2544.

Abstract

The pyrrolophenanthridine alkaloid lycorine has frequently been used as a specific inhibitor to help elucidate the function of ascorbic acid (vitamin C) in a wide range of biological processes. It was recently reported that this function can be exercised by inhibiting the activity of L-galactono-1,4-lactone dehydrogenase, the terminal enzyme of ascorbic acid biosynthesis, although working with the purified enzyme, we have been unable to repeat this result. Here, we present a procedure for the purification and analysis of lycorine by high-performance liquid chromatography from two Crinum species and describe for the first time a method that allows the simultaneous analysis of ascorbic acid and lycorine in tissue extracts of Crinum asiaticum by micellar electrokinetic chromatography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amaryllidaceae Alkaloids*
  • Ascorbic Acid / analysis*
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, Thin Layer
  • Electrochemistry
  • Electrophoresis, Capillary / methods*
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Micelles
  • Phenanthridines / analysis
  • Phenanthridines / isolation & purification*
  • Plant Leaves / chemistry
  • Plant Roots / chemistry

Substances

  • Amaryllidaceae Alkaloids
  • Micelles
  • Phenanthridines
  • lycorine
  • Ascorbic Acid