The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs

FEMS Microbiol Lett. 1997 Nov 15;156(2):205-10. doi: 10.1111/j.1574-6968.1997.tb12728.x.

Abstract

The particulate methane monooxygenase gene pmoA, encoding the 27 kDa polypeptide of the membrane-bound particulate methane monooxygenase, was amplified by PCR from DNA isolated from a blanket peat bog and from enrichment cultures established, from the same environment, using methane as sole carbon and energy source. The resulting 525 bp PCR products were cloned and a representative number of clones were sequenced. Phylogenetic analysis of the derived amino acid sequences of the pmoA clones retrieved directly from environmental DNA samples revealed that they form a distinct cluster within representative PmoA sequences from type II methanotrophs and may originate from a novel group of acidophilic methanotrophs. The study also demonstrated the utility of the pmoA gene as a phylogenetic marker for identifying methanotroph-specific DNA sequences in the environment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Probes
  • Genes, Bacterial / genetics
  • Genetic Markers
  • Metalloproteins / genetics
  • Methane / metabolism
  • Methylococcaceae / enzymology
  • Methylococcaceae / genetics*
  • Methylococcaceae / isolation & purification*
  • Oxygenases / genetics*
  • Phylogeny
  • Polymerase Chain Reaction
  • Soil Microbiology

Substances

  • DNA Probes
  • Genetic Markers
  • Metalloproteins
  • Oxygenases
  • methane monooxygenase
  • Methane

Associated data

  • GENBANK/AF006046
  • GENBANK/AF006047
  • GENBANK/AF006048
  • GENBANK/AF006049
  • GENBANK/AF006050
  • GENBANK/AF006051