Determination of genetic diversity within the genus Bifidobacterium and estimation of chromosomal size

FEMS Microbiol Lett. 1997 Nov 15;156(2):259-64. doi: 10.1111/j.1574-6968.1997.tb12737.x.

Abstract

Pulsed-field gel electrophoresis was proven to be an efficient means of differentiating 25 strains of Bifidobacterium obtained from culture collections. XbaI, SpeI, DraI restriction enzyme profiles indicated genomic heterogeneity among strains. When seven human isolates of bifidobacteria were compared using the same methods, two individual banding patterns were obtained. However, despite its discriminatory potential, pulsed-field gel electrophoresis was shown to be of no value in taxonomic identification. Genomic sizes estimated for eight Bifidobacterium strains ranged from 1.5 Mb to 2.1 Mb.

Publication types

  • Comparative Study

MeSH terms

  • Bifidobacterium / enzymology
  • Bifidobacterium / genetics*
  • Chromosomes, Bacterial*
  • DNA, Bacterial / analysis
  • Electrophoresis, Gel, Pulsed-Field
  • Enzymes / genetics
  • Genetic Variation*
  • Genome, Bacterial
  • Humans
  • Molecular Weight
  • Restriction Mapping

Substances

  • DNA, Bacterial
  • Enzymes