Induction of Smad6 mRNA by bone morphogenetic proteins

Biochem Biophys Res Commun. 1998 Mar 6;244(1):26-9. doi: 10.1006/bbrc.1998.8200.


Members of the transforming growth factor-beta (TGF-beta) superfamily transduce signals via Smad proteins. Smad2 and Smad3 mediate TGF-beta signaling, whereas Smad1 and Smad5 transduce bone morphogenetic protein (BMP) signals. Smad4 is a common mediator required for both pathways. Smad6 and Smad7 are recently identified members in the Smad family; they inhibit the signaling activity of the other Smad proteins. Here we show that expression of the Smad6 mRNA is dramatically induced by BMP-2 or osteogenic protein-1 (OP-1)/BMP-7 in various cells. BMP-2 induced expression of Smad7 in one cell type, although much less potently than that of Smad6. Smad6 message was induced by TGF-beta 1 in TGF-beta 1-responsive Mv1Lu cells, but the induction was transient in contrast to the induction by BMPs. These results indicate that Smad6 may form a feedback loop to regulate the signaling activity of BMPs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins / pharmacology*
  • CHO Cells
  • Cell Line
  • Cricetinae
  • DNA-Binding Proteins / biosynthesis*
  • DNA-Binding Proteins / drug effects
  • DNA-Binding Proteins / genetics*
  • Gene Expression Regulation / drug effects
  • Humans
  • Ligands
  • Lung
  • Mice
  • Mice, Inbred C3H
  • Mink
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / drug effects
  • Smad6 Protein
  • Trans-Activators*
  • Transforming Growth Factor beta / pharmacology


  • BMP2 protein, human
  • Bmp2 protein, mouse
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins
  • DNA-Binding Proteins
  • Ligands
  • RNA, Messenger
  • SMAD6 protein, human
  • Smad6 Protein
  • Smad6 protein, mouse
  • Trans-Activators
  • Transforming Growth Factor beta