The role of the mouse macrophage scavenger receptor in myelin phagocytosis

Eur J Neurosci. 1997 Dec;9(12):2650-7. doi: 10.1111/j.1460-9568.1997.tb01694.x.


Myelin phagocytosis during Wallerian degeneration and immune-mediated demyelination depends on the action of mononuclear cells of the monocyte/macrophage system. The present study investigated the role of the macrophage scavenger receptor, a trimeric membrane glycoprotein, in myelin uptake by macrophages. Two in vitro models of myelin phagocytosis were used: an organ culture model of mouse peripheral nerves exposed to cocultured macrophages and a quantitative flow cytometric assay. Different concentrations of the monoclonal rat anti-mouse scavenger receptor antibody (2F8) were applied to these systems to selectively block the macrophage scavenger receptor. Concentration-dependent effects on macrophage migration and myelin uptake were seen when the macrophage scavenger receptor was blocked by the antibody 2F8. Low concentrations reduced myelin phagocytosis by the invading macrophages; higher concentrations completely abolished macrophage invasion of the nerves. Using a quantitative flow cytometric assay it was also shown that the 2F8 antibody inhibits phagocytosis of myelin in a dose-dependent manner. These data indicate that the macrophage scavenger receptor is involved in myelin phagocytosis by macrophages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • Biological Transport / immunology
  • Cell Adhesion Molecules / immunology
  • Cell Adhesion Molecules / metabolism
  • Cells, Cultured
  • Demyelinating Diseases / immunology*
  • Dose-Response Relationship, Immunologic
  • Female
  • Flow Cytometry
  • Macrophages, Peritoneal / chemistry
  • Macrophages, Peritoneal / cytology
  • Macrophages, Peritoneal / immunology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Myelin Sheath / immunology*
  • Phagocytosis / immunology*
  • Receptors, Immunologic / immunology*
  • Receptors, Immunologic / metabolism
  • Receptors, Scavenger
  • Sciatic Nerve / cytology
  • Sciatic Nerve / immunology
  • Wallerian Degeneration / immunology*


  • Antibodies, Monoclonal
  • Cell Adhesion Molecules
  • Receptors, Immunologic
  • Receptors, Scavenger