Insulin-like growth factor-I partially attenuates colonic damage in rats with experimental colitis induced by oral dextran sulphate sodium

Scand J Gastroenterol. 1998 Feb;33(2):180-90. doi: 10.1080/00365529850166923.


Background: Administration of insulin-like growth factor-I (IGF-I) results in selective growth of the gastrointestinal tract. We investigated the effects of IGF-I on the colonic damage induced by oral dextran sulphate sodium (DSS) in the rat.

Methods: Rats consumed 2% DSS in the drinking water for 10 days to induce colitis. Pumps were implanted on day 3 to deliver IGF-I for 7 days. Colonic histopathology and immunolocalization of transforming growth factor-beta1 (TGF-beta1) were assessed on day 10.

Results: Compared with the colon of vehicle-treated rats consuming DSS, IGF-I increased the numbers of goblet cells by 76%, reduced the proportion of lamina propria cells expressing TGF-beta1, and reduced the thickness of submucosal and muscularis externa layers by 26% and 20%, respectively.

Conclusions: We conclude that the effects of IGF-I treatment on the colonic epithelium may be mediated directly, whereas the reduced inflammation in the mucosa and submucosa may be mediated by a mechanism other than up-regulation of TGF-beta1-mediated immunosuppression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Weight / drug effects
  • Colitis, Ulcerative / chemically induced
  • Colitis, Ulcerative / drug therapy
  • Colitis, Ulcerative / metabolism
  • Colitis, Ulcerative / pathology*
  • Colon / drug effects
  • Colon / metabolism
  • Colon / pathology*
  • Dextran Sulfate / adverse effects
  • Dose-Response Relationship, Drug
  • Immunohistochemistry
  • Insulin-Like Growth Factor I / analysis
  • Insulin-Like Growth Factor I / pharmacology*
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / metabolism
  • Intestinal Mucosa / pathology*
  • Male
  • Organ Size / drug effects
  • Rats
  • Rats, Sprague-Dawley
  • Transforming Growth Factor beta / metabolism


  • Transforming Growth Factor beta
  • Insulin-Like Growth Factor I
  • Dextran Sulfate