Measuring protein-protein interactions

Curr Opin Struct Biol. 1998 Feb;8(1):119-23. doi: 10.1016/s0959-440x(98)80019-5.


The binding of one protein to another provokes a variety of biophysical changes that can then be used as a measure of the binding reaction. Optical spectroscopy, particularly fluorescence, is the most flexible technique, but surface plasmon resonance biosensors, microcalorimetry and mass spectroscopy have recently shown significant development.

Publication types

  • Review

MeSH terms

  • Binding Sites
  • Calorimetry
  • Circular Dichroism
  • Fluorescence
  • Mass Spectrometry
  • Protein Binding*
  • Proteins / chemistry
  • Proteins / metabolism*


  • Proteins