Glutamylation of centriole and cytoplasmic tubulin in proliferating non-neuronal cells

Cell Motil Cytoskeleton. 1998;39(3):223-32. doi: 10.1002/(SICI)1097-0169(1998)39:3<223::AID-CM5>3.0.CO;2-5.

Abstract

We have examined the distribution of glutamylated tubulin in non-neuronal cell lines. A major part of centriole tubulin is highly modified on both the alpha- and beta-tubulin subunits, whereas a minor part of the cytoplasmic tubulin is slightly modified, on the beta-tubulin only. Furthermore, we observed that tubulin glutamylation varies during the cell cycle: an increase occurs during mitosis on both centriole and spindle microtubules. In the spindle, this increase appears more obvious on the pole-to-pole and kinetochore microtubules than on the astral microtubules. The cellular pattern and the temporal variation of this post-translational modification contrast with other previously described tubulin modifications. The functional significance of this distribution is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Centrioles / chemistry*
  • Cytoplasm / chemistry*
  • Humans
  • Kinetochores / chemistry
  • Lymphoid Tissue
  • Mice
  • Microtubules / chemistry
  • Mitosis
  • Polyglutamic Acid / analysis*
  • Spindle Apparatus / chemistry
  • Tubulin / analysis*

Substances

  • Tubulin
  • Polyglutamic Acid