A novel orphan G protein-coupled receptor primarily expressed in the brain is localized on human chromosomal band 2q21

J Neurochem. 1998 Apr;70(4):1357-65. doi: 10.1046/j.1471-4159.1998.70041357.x.


A human hippocampus cDNA library was screened with a probe obtained from degenerate RT-PCR aimed at P2Y-homologous sequences. A positive clone, designated hip4, was identified containing an open reading frame of 1,020 bp that had been previously detected in a published genomic clone called R12. Subsequent screening of a human fetal brain cDNA library yielded a splice variant with a 1,104-bp open reading frame, which was named fb1. Both variants display the seven-transmembrane topology that is typical for G protein-coupled receptors. Probing a human multitissue northern blot revealed two brain-specific transcripts of 2.3 and 6.3 kb, respectively. Northern blot analyses with specific fragments confirmed that the two transcripts are generated by alternative polyadenylation yielding two different 3' untranslated regions. A genomic clone from the corresponding gene was isolated and mapped to human chromosomal band 2q21 by fluorescence in situ hybridization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Brain / metabolism*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 2 / genetics*
  • DNA, Complementary / genetics
  • GTP-Binding Proteins / metabolism*
  • Genome
  • Humans
  • Molecular Sequence Data
  • RNA, Messenger / metabolism
  • Receptors, Purinergic P2 / genetics*
  • Receptors, Purinergic P2 / metabolism*
  • Tissue Distribution


  • DNA, Complementary
  • RNA, Messenger
  • Receptors, Purinergic P2
  • GTP-Binding Proteins

Associated data

  • GENBANK/Y12546
  • GENBANK/Z94154
  • GENBANK/Z94155