Expression of a receptor protein tyrosine phosphatase in human glial tumors

J Neurooncol. 1998 Feb;36(3):209-17. doi: 10.1023/a:1005840420136.

Abstract

We have analyzed expression of a receptor protein tyrosine phosphatase (RPTPzeta/beta) in tissue samples from 23 human gliomas. Using the reverse transcription-polymerase chain reaction (RT-PCR) technique, we assayed for the presence or absence of mRNA transcripts encoding the intact receptor and 2 alternatively spliced forms of RPTPzeta/beta. Transcripts encoding the intact and truncated receptors were expressed in all of the lower grade gliomas (WHO grade 1-3) analyzed, but not in 55% of the grade 4 glioblastomas multiforme (GBM). However, this subset of GBMs did express an alternatively spliced secreted form comprised of only the RPTPzeta/beta extracellular domain. Our data suggests there may be a correlation between the loss of transcripts encoding the receptor forms of RPTPzeta/beta and progression from low to high grade gliomas. This work provides additional evidence for the importance of phosphatase isoform expression in human tumors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Astrocytoma / enzymology
  • Biomarkers, Tumor / biosynthesis
  • Child
  • Female
  • Glioblastoma / enzymology
  • Glioma / enzymology*
  • Humans
  • Male
  • Middle Aged
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / genetics
  • Oligodendroglioma / enzymology
  • Protein Tyrosine Phosphatases / biosynthesis*
  • Protein Tyrosine Phosphatases / genetics
  • RNA, Messenger / biosynthesis
  • Receptor-Like Protein Tyrosine Phosphatases, Class 5
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Cell Surface / genetics

Substances

  • Biomarkers, Tumor
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • PTPRZ1 protein, human
  • Protein Tyrosine Phosphatases
  • Receptor-Like Protein Tyrosine Phosphatases, Class 5