Sequence-independent Method for in Vitro Generation of Nested Deletions for Sequencing Large DNA Fragments

Anal Biochem. 1998 Apr 10;258(1):138-41. doi: 10.1006/abio.1997.2591.

MeSH terms

  • Bacteriophage T7 / genetics
  • Base Sequence
  • Cloning, Molecular / methods
  • DNA / genetics*
  • DNA / isolation & purification
  • DNA Primers / genetics
  • Deoxyribonuclease I
  • Polymerase Chain Reaction / methods
  • Promoter Regions, Genetic
  • Sequence Analysis, DNA / methods*
  • Sequence Deletion*

Substances

  • DNA Primers
  • DNA
  • Deoxyribonuclease I