A tRNA(Glu) gene from the hyperthermophilic archaeon Pyrococcus furiosus contains the 3'-terminal CCA sequence of the mature tRNA

FEMS Microbiol Lett. 1998 Mar 15;160(2):199-204. doi: 10.1111/j.1574-6968.1998.tb12911.x.

Abstract

We cloned a gene encoding tRNA(Glu) of the hyperthermophilic archaeon Pyrococcus furiosus. This gene contains the CCA sequence corresponding to the 3'-terminus of the mature tRNA. It is known that, like in eukaryal tRNAs, the CCA-termini of archaeal tRNAs are generally not encoded. Therefore, we analyzed all tRNA genes in the genome of Methanococcus jannaschii estimated by its whole genome sequence. Twenty-one of 37 listed tRNA genes contained the 3'-terminal CCA sequence. The corresponding M. jannaschii tRNA(Glu) gene does not contain the CCA sequence, although the tRNA sequences of the M. jannaschii and P. furiosus tRNA(Glu) genes are 86% identical.

MeSH terms

  • Anticodon
  • Archaea / genetics
  • Base Sequence
  • Cloning, Molecular
  • Genes, Archaeal*
  • Methanococcus / genetics*
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Pyrococcus / genetics*
  • RNA Nucleotidyltransferases
  • RNA, Archaeal / chemistry
  • RNA, Archaeal / genetics
  • RNA, Transfer, Glu / chemistry
  • RNA, Transfer, Glu / genetics*
  • Sequence Analysis, DNA

Substances

  • Anticodon
  • RNA, Archaeal
  • RNA, Transfer, Glu
  • RNA Nucleotidyltransferases

Associated data

  • GENBANK/AB005747