Cloning, expression and mapping of a novel RING-finger gene (RNF5), a human homologue of a putative zinc-finger gene from Caenorhabditis elegans

Cytogenet Cell Genet. 1997;79(1-2):114-7. doi: 10.1159/000134695.


The RING-finger is a unique zinc-chelating domain involved in mediating protein-protein interactions. The extensive sequence homology within the RING-finger domain allowed us to clone a novel member of the RING-finger family of genes. This cDNA clone, designated RNF5 (Ring-finger protein 5), contained an open reading frame of 540 nucleotides. Its predicted amino acid sequence revealed significant homology to a hypothetical protein encoded by Caenorhabditis elegans cosmid C16C10.7. The expression of RNF5 was detected in a variety of human tissues. The RNF5 gene was mapped by fluorescence in situ hybridization to chromosome 6p21.31. Radiation hybrid mapping further assigned RNF5 to a region proximal to the major histocompatibility complex (MHC) on chromosome 6. RNF5 is the third RING-finger gene identified in the region proximal to MHC raising the possibility that the RING-finger family of genes may exist as a cluster in this region.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Caenorhabditis elegans / genetics*
  • Chromosome Mapping
  • Cloning, Molecular
  • Cosmids
  • DNA, Complementary
  • DNA-Binding Proteins / genetics*
  • Humans
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • Ubiquitin-Protein Ligases
  • Zinc Fingers / genetics*


  • DNA, Complementary
  • DNA-Binding Proteins
  • RNF5 protein, human
  • Ubiquitin-Protein Ligases

Associated data

  • GENBANK/Z46787