Preferential expression of the cDNA encoding the proteasome subunit during the growth/differentiation transition of Dictyostelium cells

Biochem Biophys Res Commun. 1998 Apr 7;245(1):231-4. doi: 10.1006/bbrc.1998.8306.

Abstract

A proteasome subunit-1 gene (DAPS-1) was isolated as one preferentially expressed during the transition from growth to differentiation in Dictyostelium discoideum cells, using the differential display method. The DAPS-1 cDNA sequence with a length of 882 bp encodes a protein (Mr. 23.4 kDa) consisting of 213 amino acids. The deduced amino acid sequence of DAPS-1 showed 61% and 58% identity to the proteasome subunit Y of Xenopus laevis and Homo sapiens, respectively and 48% and 47% identity to the proteasome subunit LMP2 of Homo sapiens and Orizas latipes, respectively. Northern analysis revealed that a 1.0 kb of DAPS-1 mRNA is predominantly expressed during the early stage of differentiation induced by starvation. This seems to indicate that the DAPS-1 protein may be involved in proteolysis coupled with active exchange of the cellular protein composition during the phase-shift of Dictyostelium cells from the proliferative to differentiated state.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Differentiation / genetics*
  • Cloning, Molecular
  • Cysteine Endopeptidases / chemistry*
  • Dictyostelium / growth & development*
  • Gene Expression Regulation, Developmental / genetics*
  • Molecular Sequence Data
  • Multienzyme Complexes / chemistry*
  • Proteasome Endopeptidase Complex
  • RNA, Messenger / metabolism
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • Multienzyme Complexes
  • RNA, Messenger
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex

Associated data

  • GENBANK/AB007024