Activation of a plant plasma membrane Ca2+ channel by TGalpha1, a heterotrimeric G protein alpha-subunit homologue

FEBS Lett. 1998 Mar 6;424(1-2):17-21. doi: 10.1016/s0014-5793(98)00129-x.

Abstract

Wild-type and GTPase-deficient recombinant TGalpha1 were used along patch-clamp techniques to study the role of heterotrimeric G proteins in the regulation of the hyperpolarized active tomato plasma membrane Ca2+ channel. Recombinant alpha-subunits induced an increase in channel activity as shown by the increase in channel events and the mean open probability of the channel. Our results suggest a membrane-delimited pathway involving heterotrimeric G proteins in Ca2+ channel activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Calcium Channels / metabolism*
  • Cell Membrane / physiology
  • GTP-Binding Proteins / metabolism*
  • Guanosine Triphosphate / metabolism
  • Lycopersicon esculentum
  • Molecular Sequence Data
  • Plant Proteins / metabolism*
  • Recombinant Proteins / immunology
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Signal Transduction

Substances

  • Calcium Channels
  • Plant Proteins
  • Recombinant Proteins
  • Guanosine Triphosphate
  • GTP-Binding Proteins