Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)

Microbiology. 1998 Jan;144 ( Pt 1):193-9. doi: 10.1099/00221287-144-1-193.

Abstract

Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single approximately 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Bacterial*
  • DNA Primers
  • DNA, Bacterial / biosynthesis
  • DNA, Bacterial / isolation & purification
  • Genes, Bacterial*
  • Ionophores / metabolism
  • Molecular Weight
  • Peptide Synthases / genetics*
  • Peptide Synthases / metabolism
  • Peptides
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Sequence Alignment
  • Streptomyces / genetics*
  • Streptomyces / metabolism*
  • Transformation, Bacterial

Substances

  • CDA antibiotic
  • DNA Primers
  • DNA, Bacterial
  • Ionophores
  • Peptides
  • Peptide Synthases