A new family of orphan G protein-coupled receptors predominantly expressed in the brain

FEBS Lett. 1998 Mar 13;424(3):193-6. doi: 10.1016/s0014-5793(98)00170-7.

Abstract

The cloning of a cDNA encoding a G protein-coupled receptor homologous to the endothelin type B receptor, but unable to bind endothelin, was recently reported and termed ET(B)R-LP. We report here the isolation of a human cDNA encoding a receptor that is highly related to ET(B)R-LP and which was therefore termed ET(B)R-LP-2. Comparison of the two amino acid sequences revealed 68% overall homology and 48% identity. As is the case for ET(B)R-LP, the new receptor is strongly expressed in the human central nervous system (e.g. in cerebellar Bergmann glia, cerebral cortex, internal capsule fibers). Membranes of HEK-293 cells stably expressing ET(B)R-LP-2 did not bind endothelin-1, endothelin-2, endothelin-3, bombesin, cholecystokinin-8 or gastrin-releasing peptide.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding, Competitive
  • Blotting, Northern
  • Brain / metabolism*
  • Central Nervous System / metabolism
  • DNA, Complementary / isolation & purification
  • Endothelins / metabolism
  • GTP-Binding Proteins / metabolism*
  • Humans
  • Molecular Sequence Data
  • Proteins / genetics*
  • Proteins / metabolism*
  • Receptor Protein-Tyrosine Kinases / genetics*
  • Receptor Protein-Tyrosine Kinases / metabolism*
  • Receptor, Endothelin B
  • Receptors, Endothelin / genetics
  • Receptors, Endothelin / metabolism
  • Receptors, G-Protein-Coupled
  • Sequence Homology, Amino Acid
  • Tissue Distribution

Substances

  • DNA, Complementary
  • Endothelins
  • GPR37L1 protein, human
  • Proteins
  • Receptor, Endothelin B
  • Receptors, Endothelin
  • Receptors, G-Protein-Coupled
  • Receptor Protein-Tyrosine Kinases
  • GTP-Binding Proteins

Associated data

  • GENBANK/Y16280