Abstract
The cloning of a cDNA encoding a G protein-coupled receptor homologous to the endothelin type B receptor, but unable to bind endothelin, was recently reported and termed ET(B)R-LP. We report here the isolation of a human cDNA encoding a receptor that is highly related to ET(B)R-LP and which was therefore termed ET(B)R-LP-2. Comparison of the two amino acid sequences revealed 68% overall homology and 48% identity. As is the case for ET(B)R-LP, the new receptor is strongly expressed in the human central nervous system (e.g. in cerebellar Bergmann glia, cerebral cortex, internal capsule fibers). Membranes of HEK-293 cells stably expressing ET(B)R-LP-2 did not bind endothelin-1, endothelin-2, endothelin-3, bombesin, cholecystokinin-8 or gastrin-releasing peptide.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Binding, Competitive
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Blotting, Northern
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Brain / metabolism*
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Central Nervous System / metabolism
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DNA, Complementary / isolation & purification
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Endothelins / metabolism
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GTP-Binding Proteins / metabolism*
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Humans
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Molecular Sequence Data
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Proteins / genetics*
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Proteins / metabolism*
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Receptor Protein-Tyrosine Kinases / genetics*
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Receptor Protein-Tyrosine Kinases / metabolism*
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Receptor, Endothelin B
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Receptors, Endothelin / genetics
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Receptors, Endothelin / metabolism
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Receptors, G-Protein-Coupled
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Sequence Homology, Amino Acid
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Tissue Distribution
Substances
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DNA, Complementary
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Endothelins
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GPR37L1 protein, human
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Proteins
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Receptor, Endothelin B
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Receptors, Endothelin
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Receptors, G-Protein-Coupled
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Receptor Protein-Tyrosine Kinases
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GTP-Binding Proteins