Phylogenetic characterization of the bacterium-like organism associated with marginal chlorosis of strawberry and proposition of a Candidatus taxon for the organism, 'Candidatus phlomobacter fragariae'

Int J Syst Bacteriol. 1998 Jan;48 Pt 1:257-61. doi: 10.1099/00207713-48-1-257.


Marginal chlorosis is a new disease of strawberry which was first seen in France in 1988. A phloem-restricted bacterium-like organism was found associated with the disease. Even though the organism could not be cultured and resembles in this way most other phloem-restricted pathogens, characterization was achieved from the sequence of its PCR-generated 16S rDNA, and comparison with other organisms. From these studies, the strawberry agent was found to be a new bacterium within group 3 of the gamma subclass of Proteobacteria, a group of Gram-negative bacteria including, in particular, insect symbionts or parasites as well as enterobacteria. Its closest relative, Arsenophonus nasoniae, is the causal agent of the son-killer trait in wasps. The two bacteria share 92% 16S rDNA sequence identity. We propose a Candidatus taxon for the marginal-chlorosis-associated bacterium, 'Candidatus Phlomobacter fragariae'.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fruit / microbiology*
  • Gram-Negative Bacteria / classification*
  • Gram-Negative Bacteria / genetics
  • Gram-Negative Bacteria / isolation & purification
  • Molecular Sequence Data
  • Phylogeny
  • Plant Diseases / microbiology*
  • Polymerase Chain Reaction
  • RNA, Bacterial / analysis
  • RNA, Ribosomal, 16S / analysis
  • Sequence Analysis, DNA


  • RNA, Bacterial
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/U91515