Molecular cloning, structural characterization, and chromosomal mapping of the human LECT2 gene

Genomics. 1998 Mar 15;48(3):324-9. doi: 10.1006/geno.1997.5198.


We originally isolated LECT2 (leukocyte cell-derived chemotaxin 2) as a 16-kDa secreted protein having a human neutrophil chemotactic activity, then cloned human and bovine LECT2 cDNAs and demonstrated the liver-specific expression of the protein. LECT2 is thought to be a multifunctional protein, because it was recently found to be identical to chondromodulin-II a growth stimulator of chondrocyte cells. We report here the cloning and the structural analysis of the human LECT2 gene. The gene spans approximately 8 kb and consists of four exons and three introns. Primer extension analysis revealed that several transcription initiation sites occur within 70-230 nucleotides upstream of the translation initiation codon. Several transcriptional control sequences relevant to the liver-specific expression have been identified at the 5' untranslated region of the human LECT2 gene. The human LECT2 gene was mapped to chromosome 5q31.1-q32 by fluorescence in situ hybridization. This region contains a cluster of cytokine genes including IL-4, IL-5, and IL-9.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites
  • Cell Line
  • Chemotactic Factors / chemistry
  • Chemotactic Factors / genetics*
  • Chemotaxis, Leukocyte
  • Chromosome Mapping
  • Chromosomes, Human, Pair 5
  • Cloning, Molecular
  • HL-60 Cells
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Molecular Sequence Data
  • Polymorphism, Genetic
  • Proteins / chemistry
  • Proteins / genetics*
  • Transcription Factors / metabolism
  • Transcription, Genetic


  • Chemotactic Factors
  • Intercellular Signaling Peptides and Proteins
  • LECT2 protein, Bos taurus
  • LECT2 protein, human
  • Proteins
  • Transcription Factors

Associated data

  • GENBANK/AB007546