Cloning and sequence analysis of the cDNA encoding the elongation factor-1 beta of Dictyostelium discoideum

Biochim Biophys Acta. 1998 Mar 3;1383(1):1-3. doi: 10.1016/s0167-4838(97)00220-3.

Abstract

A cDNA encoding elongation factor-1 beta (DdEF-1 beta) for protein synthesis was isolated from Dictyostlium discoideum cells by the differential display (DD) method. The DdEF-1 beta cDNA sequence with a length of 733 bp encodes a protein (M(r) 24.1 kDa) consisting of 214 amino acids. Comparison of the predicted amino acid sequence revealed relatively high homology to that of DdEF-1 beta from silkworm (59%), human (57%) and Xenopus (55%). Northern analysis showed that the DdEF-1 beta mRNA is maximally expressed during the vegetative growth phase, followed by marked decrease in response to cell differentiation induced by starvation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Bombyx
  • Cloning, Molecular / methods*
  • DNA, Complementary / genetics*
  • DNA, Fungal / genetics*
  • Dictyostelium / genetics*
  • Fungal Proteins / genetics*
  • Genetic Code
  • Humans
  • Molecular Sequence Data
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors / genetics*
  • Sequence Homology, Amino Acid
  • Xenopus

Substances

  • DNA, Complementary
  • DNA, Fungal
  • Fungal Proteins
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors

Associated data

  • GENBANK/AB007025