Up-regulation of p27Kip1 correlates inversely with anchorage-independent growth of human cancer cell lines

Jpn J Cancer Res. 1998 Feb;89(2):110-5. doi: 10.1111/j.1349-7006.1998.tb00537.x.


We examined the correlation between anchorage-independent growth and cell cycle-related molecules using 39 human cancer cell lines. They consisted of lung-, colon-, stomach-, breast-, ovarian-, brain-, renal- and melanoma-derived cell lines. Their anchorage-independent growth ability varied, but was not clearly related to the tissue of origin. There was a tendency for the levels of cyclin D1, cyclin E, cyclin A, p27, and p21 to show a tissue-dependent expression pattern. Statistical analysis revealed an inverse correlation of the p27 level with anchorage-independent growth (r=-0.456, P<0.01). Thus, the regulation of p27 is suggested to be linked to the anchorage independence of human cancer cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Adhesion / physiology
  • Cell Cycle / physiology
  • Cell Cycle Proteins*
  • Cell Division / physiology
  • Cyclin-Dependent Kinase Inhibitor p27
  • Humans
  • Microtubule-Associated Proteins / metabolism*
  • Neoplasms / metabolism*
  • Neoplasms / pathology*
  • Tumor Cells, Cultured
  • Tumor Suppressor Proteins*
  • Up-Regulation


  • Cell Cycle Proteins
  • Microtubule-Associated Proteins
  • Tumor Suppressor Proteins
  • Cyclin-Dependent Kinase Inhibitor p27