Molecular Cloning of leukotactin-1: A Novel Human Beta-Chemokine, a Chemoattractant for Neutrophils, Monocytes, and Lymphocytes, and a Potent Agonist at CC Chemokine Receptors 1 and 3

J Immunol. 1997 Dec 1;159(11):5201-5.

Abstract

A new member of human beta-chemokine cDNA was isolated and named leukotactin-1 (Lkn-1). Lkn-1, along with murine macrophage inflammatory protein-related protein-1 and -2, defines a subgroup of beta-chemokines based on two conserved cysteines in addition to the four others conserved in all beta-chemokines. The putative mature Lkn-1 is composed of 92 amino acids with a calculated m.w. of 10,162. The Lkn-1 gene was mapped to human chromosome 17, region q12. Recombinant Lkn-1 was a potent chemoattractant for neutrophils, monocytes, and lymphocytes and induced calcium flux in these cells. Lkn-1 specifically induced calcium flux in CCR1- and CCR3-expressing HOS cell lines. Lkn-1 suppressed colony formation by human granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors. Hence, we have isolated and characterized a human C6 beta-chemokine that is a potent agonist at CCR1 and CCR3 and shows broad biologic activities, including leukocyte chemoattraction.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Calcium / metabolism
  • Chemokines, CC / genetics*
  • Chemokines, CC / immunology
  • Chemotaxis
  • Chromosome Mapping
  • Chromosomes, Human, Pair 17
  • Cloning, Molecular
  • Humans
  • Leukocytes / immunology*
  • Lymphocytes / immunology
  • Lymphocytes / metabolism
  • Molecular Sequence Data
  • Monocytes / immunology
  • Monocytes / metabolism
  • Neutrophils / immunology
  • Neutrophils / metabolism
  • Receptors, Chemokine / antagonists & inhibitors*
  • Recombinant Proteins / genetics
  • Time Factors

Substances

  • Chemokines, CC
  • Receptors, Chemokine
  • Recombinant Proteins
  • Calcium

Associated data

  • GENBANK/U58914