Okadaic acid induces appearance of the mitotic epitope MPM-2 in SV40-infected CV-1 cells with a >G2-phase DNA content

Cytometry. 1998 Apr 1;31(4):260-4.


Simian virus 40 (SV40) infection of quiescent monkey kidney cells stimulates two successive rounds of cellular DNA synthesis without an intervening mitosis. This uncoupling of S phase and mitosis indicates that SV40 modulates pathways regulating the G2-to-M phase transition. To examine the integrity of mitotic initiation pathways in infected cells that have bypassed mitosis, SV40-infected CV-1 cells were treated with okadaic acid (OA), a known inducer of premature mitosis in other cell types. OA treatment triggered the appearance of the mitotic marker MPM-2 in SV40-infected CV-1 cells progressing through either the first (diploid) or second (tetraploid) S phases. These results demonstrate that a subset of mitotic pathways are intact but inactive in SV40-infected cells that have bypassed mitosis and initiated tetraploid S phase.

MeSH terms

  • Animals
  • Cell Line
  • Chlorocebus aethiops
  • DNA / genetics
  • DNA / metabolism*
  • Epitopes / biosynthesis
  • Flow Cytometry
  • G2 Phase / drug effects*
  • Mitosis / drug effects
  • Okadaic Acid / pharmacology*
  • Phosphoproteins / biosynthesis*
  • Phosphoproteins / immunology
  • Polyploidy
  • Simian virus 40 / pathogenicity
  • Simian virus 40 / physiology


  • Epitopes
  • Phosphoproteins
  • Okadaic Acid
  • DNA