Chemical imaging of nucleic acids, proteins and lipids of a single living cell. Application of synchrotron infrared microspectrometry in cell biology

Cell Mol Biol (Noisy-le-grand). 1998 Feb;44(1):9-13.

Abstract

Hybridoma B-cells have been used as models to evaluate the performance of synchrotron infrared microscopy to obtain chemical images of a single living cell. Chemical mapping of nucleic acids, proteins and lipids at a resolution of a few microns, close to the diffraction limit in the mid-infrared region are shown.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Lipids / analysis*
  • Mice
  • Nucleic Acids / analysis*
  • Proteins / analysis*
  • Spectrophotometry, Infrared / methods*
  • Synchrotrons
  • Tumor Cells, Cultured

Substances

  • Lipids
  • Nucleic Acids
  • Proteins