Molecular characterization of NOS in a mollusc: expression in a giant modulatory neuron

J Neurobiol. 1998 Apr;35(1):65-76. doi: 10.1002/(sici)1097-4695(199804)35:1<65::aid-neu6>;2-9.


Here we report on the molecular characterization of the first molluscan NOS in the CNS of the pond snail Lymnaea stagnalis. This Lymnaea NOS (Lym-nNOS) which is expressed preferentially in the CNS is most similar to mammalian neuronal NOS but contains tandem repeats of a seven amino acid motif not found in any other known NOS. We have localized Lym-nNOS to the serotonergic cerebral giant cells (CGCs) which modulate synaptic transmission within a neural network that generates feeding behavior. Our results suggest that the CGCs employ both NO and serotonin in the modulation of the central neural network underlying feeding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aplysia / enzymology*
  • Base Sequence
  • Calcium / physiology
  • Cell Size
  • Central Nervous System / enzymology
  • Cloning, Molecular
  • Lymnaea / enzymology*
  • Molecular Sequence Data
  • Neurons / cytology
  • Neurons / enzymology*
  • Nitric Oxide Synthase / genetics*
  • RNA, Messenger / metabolism
  • Tissue Distribution


  • RNA, Messenger
  • Nitric Oxide Synthase
  • Calcium

Associated data

  • GENBANK/AF012531