Locally recurrent prostate tumors following either radiation therapy or radical prostatectomy have changes in Ki-67 labeling index, p53 and bcl-2 immunoreactivity

J Urol. 1998 May;159(5):1437-43. doi: 10.1097/00005392-199805000-00004.


Purpose: We compare the biological phenotype of recurrent prostatic tumors after definitive local therapy (radiation or radical prostatectomy) with that of the same tumors before treatment.

Materials and methods: Cellular proliferation (Ki-67 labeling index), p53 nuclear reactivity and bcl-2 immunoreactivity were determined in pretreatment and posttreatment tumor specimens from 13 patients with local tumor recurrence following radiation, and in 18 patients with local tumor recurrence following radical prostatectomy.

Results: Mean Ki-67 labeling index increased approximately 2-fold in locally recurrent tumors after radiation (10.5 versus 5.6%, p=0.0008) or surgery (6.0 versus 3.2%, p=0.0025) when compared with pretreatment tumors. We noted p53 nuclear reactivity in a significantly higher proportion of recurrences than in pretreatment tumors following radiation (54 versus 8%, p=0.032) and surgery (39 versus 5%, p=0.022). Although bcl-2 immunoreactivity was also seen in a higher proportion of recurrent tumors, this difference did not reach statistical significance for either radiation or surgery.

Conclusions: Recurrent tumors following either radiation or surgery differ significantly from the corresponding pretreatment tumors with respect to cellular proliferation and p53 nuclear reactivity.

MeSH terms

  • Adenocarcinoma / metabolism*
  • Adenocarcinoma / radiotherapy
  • Adenocarcinoma / surgery
  • Aged
  • Cell Division
  • Humans
  • Immunoenzyme Techniques
  • Ki-67 Antigen / biosynthesis*
  • Male
  • Middle Aged
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Recurrence, Local / metabolism*
  • Nuclear Proteins / biosynthesis*
  • Prostatectomy
  • Prostatic Neoplasms / metabolism*
  • Prostatic Neoplasms / radiotherapy
  • Prostatic Neoplasms / surgery
  • Proto-Oncogene Proteins c-bcl-2 / biosynthesis*
  • Tumor Suppressor Protein p53 / biosynthesis*


  • Ki-67 Antigen
  • Neoplasm Proteins
  • Nuclear Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Tumor Suppressor Protein p53