In situ visualization of DNA double-strand break repair in human fibroblasts

Science. 1998 Apr 24;280(5363):590-2. doi: 10.1126/science.280.5363.590.

Abstract

A method was developed to examine DNA repair within the intact cell. Ultrasoft x-rays were used to induce DNA double-strand breaks (DSBs) in defined subnuclear volumes of human fibroblasts and DNA repair was visualized at those sites. The DSBs remained in a fixed position during the initial stages of DNA repair, and the DSB repair protein hMre11 migrated to the sites of damage within 30 minutes. In contrast, hRad51, a human RecA homolog, did not localize at sites of DNA damage, a finding consistent with the distinct roles of these proteins in DNA repair.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bromodeoxyuridine / immunology
  • Bromodeoxyuridine / metabolism
  • Cell Line
  • Cell Nucleus / metabolism*
  • DNA / metabolism*
  • DNA / radiation effects
  • DNA Damage*
  • DNA Repair*
  • DNA-Binding Proteins / metabolism
  • Fibroblasts
  • Fluorescein-5-isothiocyanate
  • Fluorescent Antibody Technique
  • Humans
  • Microscopy, Confocal
  • Rad51 Recombinase

Substances

  • DNA-Binding Proteins
  • DNA
  • RAD51 protein, human
  • Rad51 Recombinase
  • Bromodeoxyuridine
  • Fluorescein-5-isothiocyanate